Assistant Professor (May 2013-): Biological Sciences, Indian Institute of Science, Education and Research (IISER) Bhopal
Postdoctoral Fellow (March 2010-March 2013): National Cancer Institute, National Institutes of Health, Maryland, USA
Postdoctoral Fellow (May 2009-Feb 2010): Georgetown University Medical Centre, Washington DC, USA
Ph.D. (Nov 2003-Feb 2009): Advanced Centre for Treatment, Research and Education in Cancer (CRI-ACTREC), Navi Mumbai
I am looking for postdoctoral candidate who is interested to study epigenetic regulation of alternative pre-mRNA splicing/RNA processing in tumorigenesis. Interested candidate can send their cv and statement of research interest by email to me at email@example.com
Epigibility: Candidate should have PhD in biological sciences from recognized university/institution.
Desirable: Prior experience with molecular biology techniques. Candidates with experience in analysis of next-generation sequencing data/ RNA work / chromatin biology are encouraged to apply.
-Examination of DNA methylation mediated regulation of alternative splicing via modulation of reciprocal binding of CTCF and BORIS in cancer cells.
-Analysis of DNA binding proteins/ transcription factors and upstream pathways that are involved in regulation of alternative splicing
-Examination of role of cancer specific alternative spliced isoforms in tumorigenesis
Research Interests: Epigenetics and RNA Processing
Interplay between epigenetic changes and alternative pre-mRNA splicing in tumorigenesis
-Shukla S, Govekar RB, Sirdeshmukh R, Sundaram CS, D’Cruz A, Pathak KA, Kane SV, Zingde SM Tumor antigens eliciting autoantibody response in cancer of gingivo-buccal complex. Proteomics Clinical Applications 2007, 1(12): 1592-1604.
-Shukla S, Pranay A, D’Cruz AK, Chaturvedi P, Kane SV, Zingde SM “Immunoproteomics reveals that cancer of the tongue and the gingivo buccal complex exhibit differential autoantibody response.” Cancer Biomarkers 2009, 5(3): 127-35.
Received F1000 article factor (FFa) -17 (exceptional rating) from Faculty of 1000 based on following 4 evaluations. http://f1000.com/13371987
This article was also highlighted in CCR connections Vol 6 (1), 2012 “CTCF, a Novel Regulator of Alternative Splicing” http://home.ccr.cancer.gov/connections/ dev/2012/Vol6_No1/news_7.asp
This article was highlighted in Cell Research- Kornblihtt A. “CTCF: from insulators to alternative splicing regulation”, Cell Research advance online publication 7 February 2012; doi:10.1038/cr.2012.22 http://www.nature.com/cr/journal/vaop/ncurrent/full/cr201222a.html
-Shukla S, Kavak E, Gregory M, Imashimizu M, Shutinoski B, Kashlev M, Oberdoerffer P, Sandberg R, Oberdoerffer S. “CTCF-promoted RNA polymerase II pausing links DNA methylation to splicing”. Nature 479, 74–79 (03 November 2011).
-Shukla S., Oberdoerffer S. Co-transcriptional regulation of alternative pre- mRNA splicing. BBA-Gene Regulatory Mechanism July 2012, 1819 (7), 673- 683.
-Pranay A, Shukla S et al. Prognostic utility of autoantibodies to α-enolase and Hsp70 for cancer of the gingivo-buccal complex using immunoproteomics. Proteomics Clinical Applications 2012; DOI: 10.1002/prca.201200081.
Zingde, Shukla, Sirdeshmukh, Sundaram D’Cruz, Pathak, Kane [Inventors] Autoantibodies for protein antigens as markers for cancer of the ginigivo –buccal complex:
CSIR: Ref No: 0187NF 2006/1N, filed on Feb 14, 2007; 290DEL 2007
European Patent Application EP2126578 (Published: Feb 12, 2009)
US application- US 2011/0189699A1 (published: date Aug 04, 2011)